Anticancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) capped silver and zinc oxide nanoparticles and its molecular characterization
Anticancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) capped silver and zinc oxide nanoparticles and its molecular characterization
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Abstract The current investigation reports anti-cancer, antioxidant and antibacterial potential of L-Glutaminase (Streptomyces roseolus strain ZKB1) and L-Glutaminase capped nanoparticles.The highest L-Glutaminase production of 9.57 U/mL was achieved on the 4th day of fermentation when L-Glutamine was used as the sole carbon and nitrogen source.Enhanced recycling stability here was observed after 6 cycles using L-Glutaminase immobilized in 3% agar and agarose matrices.
Free and immobilized L- Glutaminase showed K m of 13.89 ± 0.8 and 7.13 ± 0.
3 mM and V max of 18.40 ± 1.5 and 24.21 ± 1.
7 U/mg respectively.L- Glutaminase capped silver (AgNP) and zinc oxide (ZnONP) nanoparticles were synthesized and structurally characterized using UV visible spectroscopy, FTIR, SEM–EDS, XRD and AFM.L- Glutaminase capped AgNP and ZnONP exhibited good thermal stability with five and three stages weight loss pattern respectively based on TGA.L-Glutaminase capped AgNP exhibited highest inhibitory activity against B.
subtilis (45 $$pm$$ ± 0.5 mm) and E.coli (33 $$pm$$ ± 0.8 mm) whereas, L-Glutaminase capped ZnONP demonstrated highest inhibition against E.
coli (30 $$pm$$ ± 0.3 mm) and B.cereus (25 $$pm$$ ± 0.5 mm).
Increased nanoparticles concentration exhibited click here increased inhibitory potential as compared to wild L-Glutaminase and lowest MIC of 0.09 µg/mL was exhibited against B.cereus.L-Glutaminase capped nanoparticles demonstrated significant antioxidant properties through in-vitro ABTS and DPPH radical scavenging assays in a dosage-dependent manner.
L-Glutaminase and capped AgNP and ZnONP, demonstrated pronounced cell cytotoxicity against MCF-7 cancerous cell line with 57.17 µg/mL, 8.13 µg/mL and 28.31 µg/mL IC50 values respectively, suggesting promising properties as anticancer agents in enzyme-based therapy.
The results reveal promising biological activities with potential applications in healthcare sector.Graphical Abstract.